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KMID : 0644120170300020001
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Journal of Oriental Gynecology
2017 Volume.30 No. 2 p.1 ~ p.15
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Effects of Patrinia Scabiosaefolia Aqueous Extract on Cytokine and NF-κB Activation in LPS-induced RAW 264.7 Cells and Mouse
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Ryu Ik-Han
Cho Hae-Joong
Song Mi-Hwa
Choi Chang-Min
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Abstract
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Objectives: The object of this study was to identify the anti-inflammatory effects of Patrinia scabiosaefolia aqueous extract (PSE).
Methods: RAW 264.7 cells were pre-treated with PSE and then incubated with or without lipopolysaccharide (LPS). Cell viability, production of nitric oxide (NO), secretion of pro-inflammatory cytokine, activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB) were measured. In addition, we observed mice survival rate after LPS and their cytokine levels of serum. We also observed inflammatory and hemorrhagic change on the histological sections of the liver.
Results: PSE inhibited LPS-induced NO production, interleukin (IL)-6 secretion, c-Jun NH2-terminal kinase (JNK) and NF-κB activation. In addition, PSE reduced the death rate of LPS-induced mice and IL-6 production on the serum of mice. PSE inhibited inflammation and hemorrhage on liver tissue as well.
Conclusions: The results suggest that PSE have anti-inflammatory effects by inhibited NF-κB and JNK activation, IL-6 secretion, and NO production. So PSE may be effective treatment for the inflammatory disease.
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KEYWORD
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Patrinia Scabiosaefolia (PS) , Inflammation , NO , Cytokine , MAPKs , NF-κB
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